Table 1.

Limits of detection (LoD) for home-made and commercial new extraction method (NEM) buffers using Whatman 3MM dried blood spots

Step	Home-made	Commercial
Lysis	3M Guanidine thiocyanate 16.7% Isopropanol 2% Triton X100 10 mM EDTA 5 mM Trizma HCl pH 7.4 0.1% 6 N HCl 0.5% 2-mercaptoethanol pH 6.0–6.5	Qiagen RLT-plus 16.7% Isopropanol 0.5% 2-mercaptoethanol
Wash 1	Same as lysis but no 2-mercaptoethanol	Same as lysis but no 2-mercaptoethanol
Wash 2	25% Ethanol 25% Isopropanol 100 mM Sodium chloride 10 mM Trizma HCl pH 7.4	70% Ethanol 30% PBS
LoD	20 parasites/mL	22 parasites/mL