Table 4.
Predicted housekeeping genes from the thyroid transcriptome map (explanation in the text); (n = 45)
| Gene symbol | Chromosome | Location | Expression value | Data points | SD |
|---|---|---|---|---|---|
| Hs.670607 | chr4 | N/A | 232.70 | 18 | 11.95 |
| BLOC1S2 | chr10 | 10q24.31 | 181.44 | 22 | 13.39 |
| Hs.706927 | chr6 | N/A | 357.37 | 18 | 13.82 |
| TMEM256 | chr17 | 17p13.1 | 153.91 | 22 | 18.35 |
| POM121C | chr7 | 7q11.2 | 347.50 | 20 | 22.49 |
| CUEDC2 | chr10 | 10q24.32 | 210.93 | 31 | 22.83 |
| RPLP0* | chr12 | 12q24.2 | 3,340.82 | 76 | 23.40 |
| PJA1 | chrX | Xq13.1 | 234.54 | 31 | 23.83 |
| Hs.713747 | chr1 | N/A | 169.93 | 18 | 23.92 |
| RAB25 | chr1 | 1q22 | 199.48 | 31 | 24.00 |
| CIB1 | chr15 | 15q25.3-q26 | 314.61 | 33 | 25.01 |
| MRPL18 | chr6 | 6q25.3 | 182.80 | 23 | 25.09 |
| SCAMP3 | chr1 | 1q21 | 152.98 | 33 | 25.11 |
| Hs.633993 | chr8 | N/A | 2,050.15 | 18 | 25.21 |
| Hs.714416 | chr3 | N/A | 100.67 | 18 | 25.31 |
| RBM23 | chr14 | 14q11.2 | 257.95 | 31 | 25.67 |
| Hs.117688 | chr8 | N/A | 160.32 | 22 | 25.73 |
| Hs.546523 | chr2 | N/A | 178.40 | 18 | 25.96 |
| DCXR | chr17 | 17q25.3 | 210.53 | 31 | 25.98 |
| Hs.193784 | chr3 | N/A | 491.90 | 22 | 26.02 |
| ABRACL | chr6 | 6q24.1 | 116.71 | 23 | 26.15 |
| MAP1LC3B2 | chr12 | 12q24.22 | 157.45 | 18 | 26.29 |
| DGCR6 | chr22 | 22q11 | 209.18 | 20 | 26.31 |
| GTPBP6 | chrX; chrY | Xp22.33; Yp11.32 | 120.83 | 23 | 26.61 |
| AIMP2 | chr7 | 7p22 | 181.19 | 43 | 26.84 |
| CSNK2B | chr6 | 6p21.3 | 337.74 | 25 | 26.89 |
| TM2D2 | chr8 | 8p11.22 | 256.54 | 28 | 27.09 |
| CLIC1 | chr6 | 6p21.3 | 530.89 | 25 | 27.21 |
| Hs.715132 | chrX | N/A | 196.12 | 22 | 27.42 |
| Hs.732277 | chr19 | N/A | 218.68 | 22 | 28.49 |
| Hs.705664 | chr7 | N/A | 1,167.55 | 18 | 28.62 |
| NMRAL1 | chr16 | 16p13.3 | 110.71 | 45 | 28.70 |
| Hs.713819 | chr5 | N/A | 241.81 | 18 | 28.93 |
| RPP21 | chr6 | 6p22.1 | 111.91 | 23 | 28.94 |
| PNMA1 | chr14 | 14q24.3 | 148.75 | 23 | 28.94 |
| ACTG1* | chr17 | 17q25 | 3,583.46 | 180 | 29.06 |
| AKR1B1 | chr7 | 7q35 | 258.94 | 25 | 29.09 |
| APOA1BP | chr1 | 1q21 | 231.69 | 32 | 29.35 |
| MRPL24 | chr1 | 1q23.1 | 183.95 | 31 | 29.44 |
| ABCF1 | chr6 | 6p21.33 | 123.95 | 25 | 29.56 |
| ZNF627 | chr19 | 19p13.2 | 112.37 | 24 | 29.70 |
| METRNL | chr17 | 17q25.3 | 175.03 | 22 | 29.78 |
| OCIAD2 | chr4 | 4p11 | 286.18 | 20 | 29.92 |
| EIF2B2 | chr14 | 14q24.3 | 248.05 | 33 | 29.94 |
| DIABLO | chr12 | 12q24.31 | 211.60 | 31 | 29.98 |
Genes are sorted in ascending order of SD as percentage of the mean value. *In bold, the two best genes at behaving like a housekeeping gene due to a combination of a low SD, a high expression value and a high number of data points, following checking in the “Values A” TRAM table that the 180 (ACTG1) and 76 (RPLP0) data points are derived from 33 and 22 samples, respectively, out of the 35 thyroid samples analyzed. N/A: not available in the Gene database (http://www.ncbi.nlm.nih.gov/gene) when the analysis was performed