Table 6.

Selected genes to validate the thyroid transcriptome map in vitro through Real-Time RT-PCR

Gene symbol	Gene name	EEV	SD	ER	Ct mean	OR
TG	Thyroglobulin	8,662.34	33.14	11.51	17.55	7.41
RPL41	Ribosomal protein L41	5,903.29	51.15	7.85	17.11	10.056
B2M	Beta-2-microglobulin	2,988.28	64.22	3.97	18.22	4.66
ACTB	Actin, beta	2,338.95	63.50	3.11	19.38	2.085
TPO	Thyroid peroxidase	2,245.11	54.57	2.98	19.38	2.08
IYD	Iodotyrosine deiodinase	890.07	65.51	1.18	21.16	0.61
SDC2	Syndecan 2	752.49	59.44	1	20.44	1
TSHR	Thyroid stimulating hormone receptor	487.64	91.14	0.65	21.34	0.54
SERPINF1	Serpin family F member 1	320.48	65.03	0.43	23.63	0.11
MYH9	Myosin, heavy chain 9, non-muscle	315.95	104.13	0.42	24.19	0.074
BACE2	Beta-site APP-cleaving enzyme 2	105.67	47.06	0.14	24.75	0.050
REEP3	Receptor accessory protein 3	85.88	108.64	0.11	N/A	N/A
YKT6	YKT6 v-SNARE homolog (S. cerevisiae)	29.63	96.23	0.04	26.08	0.020
NTNG1	Netrin G1	17.89	83.44	0.024	32.51	2.33 × 10−4
RIPPLY3	Ripply transcriptional repressor 3	16.88	97.44	0.022	33.10	1.55 × 10−4
NPTX1	Neuronal pentraxin 1	16.77	79.37	0.022	33.37	1.28 × 10−4
TBX18	T-box 18	11.52	100.73	0.015	28.63	0.0034

In bold the gene chosen as reference. From left to right: official gene symbol of the selected gene; official full gene name; expected expression value (EEV), i.e. mean linear expression value for the gene as provided by TRAM software; standard deviation (SD) as percentage of the mean; expected ratio (ER) between target and reference gene expression value; threshold cycle (Ct) mean provided by BioRad CFX Manager Software 2.1 manually positioning the threshold line; observed ratio (OR) determined between each target gene and the reference gene using the 2^∆Ct method, according to the formula: 2^ΔCt = 2 ^{Ct ref. - Ct target}