Table 1.

Composition of washing solutions

	Volume (ml)
Solutions	B	B + L	B + S	B + D + L	B + D + S	B + D + L + S
0.1 M Tris buffer pH = 8.5	40	40	40	40	40	40
Lipase	–	10	–	10	–	10
Biosurfactant	–	–	10	–	10	10
1% Detergent solution	–	–	–	40	40	40
Distilled water	60	50	50	10	10	–

B = 0.1 M Tris buffer pH = 8.5

L = lipase (1000 U)

S = biosurfactant (1000 U)

D = 1% Detergent solution

The stained fabrics were incubated in 250 ml flasks containing 100 ml washing solutions as mentioned below. All tests were repeated at least in triplicate independent experiments