Table 4.
Effect of 0.1% organic solvents and 25% detergents on biosurfactant and lipase of O. intermedium strain MZV101 stabilities
| Organic solvents/detergents | Emulsification index (%) | Relative activity (%) |
|---|---|---|
| Control | 100 | 100 |
| Acetone | 94.59 | 20.00 |
| Benzene | 88.23 | 77.46 |
| Chloroform | 88.98 | 44.01 |
| Cyclohexane | 87.83 | 112.67 |
| Dimethyl | 82.45 | 88.02 |
| Ethanol | 92.99 | 23.05 |
| Ethyl Acetate | 91.21 | 107.18 |
| Hexane | 86.75 | 101.01 |
| Methanol | 93.23 | 25.07 |
| Toluene | 87.56 | 131.26 |
| 1% SDS | 98.65 | 122.53 |
| Tween-20 | 92.34 | 117.74 |
| Tween- 80 | 91.23 | 80.98 |
| Triton X-100 | 85.30 | 35.11 |
The tested were studied on lipase stability of O. intermedium strain MZV101 in optimal pH 10 and in optimal temperature 60 °C incubated for 4 h and same mentioned condition was used for biosurfactant O. intermedium strain MZV101 incubated for 24 h. Results were represented as mean ± S.D. and all tests are examined at least in triplicate independent experiments