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. 2017 Aug 22;8(14):2729–2739. doi: 10.7150/jca.18396

Figure 4.

Figure 4

Validation of miR-26a-5p/BIRC5 interaction and BIRC5 function in prostate cancer. Relative quantification of BIRC5 expression level at both transcriptional (by qRT-PCR) and translational (by western blot) levels after BIRC5 silencing (A) and miR-26a-5p overexpression (C) in DU-145 cells. (B) Relative Luciferase activity after the cotransfection of pmiR-BIRC5 3' UTR and either miR-26a-5p or miR-CT. Cell proliferation, survival, apoptosis (D), cell cycle (E) and cell migration (F) in DU-145 cells after BIRC5 silencing. Data are reported as mean of at least three independent experiments (*P<0.05, **P<0.01, ***P<0.001, unpaired t-test).