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. Author manuscript; available in PMC: 2018 Aug 20.
Published in final edited form as: J Genet Genomics. 2017 Jul 25;44(8):375–383. doi: 10.1016/j.jgg.2017.04.008

Fig. 4. The effect of shGLI2 and 5Fu on ABCG2 expression.

Fig. 4

A: qPCR analysis of ABCG2 after 5Fu treatment in N87 cells. B: Flow cytometry analysis of surface ABCG2 protein expression. The average positivity from there independent experiments was shown on the right. C and D: Comparison of 5Fu resistant N87/FR cells with the parental N87 cells on ABCG2 expression (transcript level shown on the left; and cell surface expression shown on the right). E and F: The effect of GLI2 knockdown on ABCG2 transcript (left) and on cell surface expression (right). Significant difference was indicated by * (P < 0.05), ** ( P < 0.005), or *** (P < 0.0005).