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Analysis of HDGF expression by confocal immunofluorescence microscopy. Briefly, LNCaP cells cultured in tissue culture slides were probed with mouse anti-HDGF mAb antibody. HDGF expression was detected using goat anti-mouse FITC and counterstained with DAPI. Confocal image analysis suggests that HDGF is predominantly localized in nucleus as shown in the merged images of DAPI and HDGF (FITC).
