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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Urol Oncol. 2016 Sep 28;34(11):483.e1–483.e8. doi: 10.1016/j.urolonc.2016.05.027

Figure 3.

Figure 3

Ectopic expression of HDGF in RWPE-1 cells promotes cell proliferation. Over expression of HDGF is confirmed in the transfected cells by Western blot and quantification by ImageJ analysis (A &B). (C) Mock transfected (pCtrl) and RWPE-1 cells transfected with HDGF were seeded in a 96 well plate and the viability of the cells were determined at different time points. Results show that the RWPE-1 HDGF cells proliferate at approximately 180% of the rate of control RWPE-1 after 72 hours. *p<0.01 compared to control transfected cells.