Fig. 1.
Performance of the rRT-PCR assay utilising the primer combination F2 + R3. a Specificity of the assay in the presence of mosquito RNA. The mosquito RNA was spiked with a 5-fold dilution (102–10-2 PFU/ml) of ZIKV RNA, to determine any primer cross-reactivity with mosquito-derived genomic material. b Specificity of the assay in a panel of flaviviruses and alphaviruses. The melting peak for ZIKV ranged between 80.3–80.4 °C, while that of primer dimers ranged between 73.0–75.8 °C. c Amplification curves for 10-fold serial dilutions (104–10-4 PFU/ml) of ZIKV. d Standard curve for the quantification of ZIKV infectious virus particles. The curve was generated by plotting the quantification cycle (Cq) values against the log10 of infectious virus particles (PFU/ml). Only the detection range is shown. RNA from ZIKV isolate PLCal_ZV was used in the analysis