Fig. 5.

OGN activates mTOR signaling and AKT. a OGN-expressing cells demonstrate increased activation of mTOR and its downstream signaling effectors at 48 h, including p-mTOR (Ser2448), p-4E-BP1(Ser65), p-eIF4B (Ser422), and p-eEF2K (Ser366), compared to controls on Western blot analysis with densitometry quantification. b Treatment with the mTOR inhibitor rapamycin (Rapa) suppresses mTOR activation without affecting OGN expression in OGN cells, as compared to DMSO control at 6 h. c OGN-expressing cells also activate AKT (p-AKT Ser473) as early as 6 h after plating, on Western blot analysis with densitometry quantification. d Addition of rapamycin (Rapa) does not affect AKT activation at 6 h in OGN cells. *p < 0.05