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. Author manuscript; available in PMC: 2017 Sep 19.
Published in final edited form as: Cell Signal. 2014 Dec 11;27(3):453–459. doi: 10.1016/j.cellsig.2014.11.037

Fig. 3.

Fig. 3

Src is required for HMWHA-induced filamentous actin polymerization. (A–D) Tgfbr3+/+ epicardial cells were dosed without (A, B) or with Src kinase inhibitor (PP2 1 μM) (C, D) for 60 min, followed by 300 μg/mL HMWHA stimulation in the presence (D) or absence (B) of PP2 for 60 min, and subjected to phalloidin staining to visualize filamentous actin. (E) Globular (g) or filamentous (f) actin detection by Western blot following ultracentrifugation of lysates from vehicle incubated and HMWHA-stimulated epicardial cells with or without PP2. (F) Representative graph of the f/g actin ratio for HMWHA stimulated vehicle incubated epicardial cells with or without PP2.