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. 2017 Aug 25;8(15):2933–2943. doi: 10.7150/jca.20319

Figure 1.

Figure 1

CHR-6494 decreased cell viability and increased apoptosis in melanoma cells. Melanoma cells (as indicated) were plated as triplicates in 96-well plates, and treated with CHR-6494, MEK inhibitor GSK1120212 (MEKi), or DMSO (vehicle control) at various concentrations for 72 h. (A-C) Cell viability was determined with crystal violet staining assay. The y-axis represents mean-percentage of viable cells ± S.E.M, N=3 independent experiments. (D) Western blot analysis using an antibody against histone H3 Thr3 phosphorylation (H3T3P). (E) Caspase 3/7 activity measured using the Caspase 3/7 Glo Assay Kit. Respective values are normalized to protein concentrations and presented as mean-fold over control ± S.E.M. *, p < 0.05, **, p < 0.01, N=3. (F) Western blot analysis for the cleaved form of PARP.