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. 2017 Aug 25;8(15):2933–2943. doi: 10.7150/jca.20319

Figure 3.

Figure 3

Combination between CHR-6494 and the MEK inhibitor enhanced apoptosis in MeWo and MDA-MB-435 melanoma cell lines. MeWo and MDA-MB-435 melanoma cells or HDFs were treated with CHR-6494, MEK inhibitor GSK1120212 (MEKi), or the combination of two compounds at various concentrations for 5 days. (A-B) Cells were harvested for western blot analysis using an antibody against the cleaved form of PARP. (C) Caspase 3/7 activity was measured using the Caspase 3/7 Glo Assay Kit and normalized to protein concentrations. Respective values are presented as mean-fold over control ± S.E.M. *, p < 0.05, **, p < 0.01 ***, p < 0.001, N=3.