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. 2017 Sep 2;8(15):3037–3048. doi: 10.7150/jca.19315

Figure 2.

Figure 2

EZH2 is a target of miR-340. (A) Diagram of seed sequence of miR-340 matched the 3'UTR of the EZH2 gene. (B) EZH2 expression was detected by western blot after miR-340 mimics and miR-340-inhibitor transfection. (C) EZH2 expression was assayed by western blot after miR-340 inhibitor transfection in MCF-7 and T47D cells. (D) Schematic diagram of EZH2 3'UTR containing reporter constructs. (E) Luciferase reporter assays in breast cancer cells, following cotransfection with the 3'UTR EZH2 and miR-340 mimics, miR-340-inhibitor, and mutant miR-340 sequence as indicated. The data represent the fold change in expression (means ± SE) of three replicates. Data represent mean ± SD of three replicates. *P < 0.05.