Figure 3.

Identification of the targets of the HMG-14 point mutants. Acetylated (ac) or nonacetylated (n) cores containing the ¹²⁵I-labeled HMG-14 point mutants indicated at the top of the lanes were exposed to UV, treated with 2-mercaptoethanol, and analyzed by SDS/PAGE. (A) Autoradiograph. (B) Coomassie blue stain. Lanes 5–8 contained only the labeled HMG-14 mutants. Lanes 7 and 8 were not exposed to UV and therefore the radioactive label runs with the ion front. (C) The degree of acetylation of the histones in the nucleosome cores was assessed by Coomassie blue staining of Triton/acid/urea polyacrylamide gels (33).