Table 1.
Orchid mycorrhizal fungi identified from roots of seven Epipactis species from nine sites in Germany and the Netherlands (ECM A = ascomycetes forming ectomycorrhizas, ECM B = basidiomycetes forming ectomycorrhizas); L is Ellenberg’s light indicator value (Ellenberg et al., 1991) and n is the number of Epipactis individuals sampled
| Species | L | Site | n | Pelotons | Mycorrhizal fungi | Type of mycorrhizal fungi | Best match sequence/ accession number (UDB-UNITE, others GenBank) | Identity (%) |
|---|---|---|---|---|---|---|---|---|
| Epipactis helleborine (L.) Crantz* | 3 | Ruderal site 1 | 1 | NA | Helotiales | ECM A | DQ182433 uncul. Helotiales | 98·9 |
| NA | Inocybe sp. | ECM B | HE601882·1 uncul. Inocybe | 99·4 | ||||
| NA | Sebacina sp. | ECM B | UDB013653 Sebacina | 99·7 | ||||
| Epipactis helleborine (L.) Crantz* | 3 | Forest site 2 | 1 | NA | Thelephoraceae | ECM B | UDB013578 Tomentella-Thelephora | 97·7 |
| NA | Helotiales | ECM A | DQ182433 uncul. Helotiales | 98·9 | ||||
| NA | Tomentella sp. | ECM B | AJ879656·1 uncul. Ectomycorrhiza (Tomentella) | 96·5 | ||||
| NA | Inocybe sp. | ECM B | JX630876 uncul. Inocybe | 98·5 | ||||
| NA | Tuber rufum | ECM A | EF362475 Tuber rufum | 100 | ||||
| NA | Inocybe sp. | ECM B | HE601882·1 uncul. Inocybe | 99·4 | ||||
| NA | Tuber sp. | ECM A | AJ510273 uncul. Tuber sp. | 99·6 | ||||
| NA | Sebacina sp. | ECM B | UDB007522 Sebacina | 99·6 | ||||
| Epipactis helleborine subsp. neerlandica (Verm.) Buttler | NA | Dune site 3 | 1 | no | Inocybe sp. | ECM B | JF908119·1 Inocybe splendens | 90 |
| Epipactis microphylla (Ehrh.) Sw. | 2 | Forest site 4 | 5 | yes | Sebacina epigaea | ECM B | KF000457·1 Sebacina epigaea | 100 |
| yes | Cadophora sp. | ECM A | JN859252·1 Cadophora sp. | 99 | ||||
| Epipactis purpurata Sm. | 2 | Forest site 5 | 5 | no | Russula heterophylla | ECM B | DQ422006·1 Russula heterophylla | 99 |
| no | Inocybe sp. | ECM B | KF679811·1 Inocybe sp. | 91 | ||||
| Epipactis distans Arv.-Touv. | NA | Forest site 6 | 5 | yes | Wilcoxina rehmii | ECM A | DQ069001·1 Wilcoxina rehmii | 99 |
| Epipactis leptochila (Godfery) Godfery | 3 | Forest site 7 | 5 | yes | Tuber excavatum | ECM A | HM151977·1 Tuber excavatum var. intermedium | 99 |
| yes | Tuber excavatum | ECM A | HM151993·1 Tuber excavatum | 99 | ||||
| Epipactis muelleri Godfery | 7 | Forest site 8 | 5 | yes | Tuber puberulum | ECM A | FN433157·1 Tuber puberulum | 100 |
| AF106891·1 Tuber oligospermum | 99 | |||||||
| Epipactis neglecta (Kümpel) Kümpel | NA | Forest site 9 | 5 | yes | Tuber excavatum | ECM A | HM151977·1 Tuber excavatum var. intermedium | 99 |
*
Data from Ion Torrent sequencing.