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. 2017 Jan 8;119(4):629–643. doi: 10.1093/aob/mcw252

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© The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Measurement of solute permeabilities (P_sr) and reflection coefficients (σ_sr) of seminal roots of barley for different test solutes using the root pressure probe in well-stirred conditions. Responses of root pressure (P_r) in relation to the change in osmotic pressure in the medium either by adding 100 mOsmol kg^–1 ethanol (A), 56 mOsmol kg^–1 KCl (B), 40 mOsmol kg^–1 mannitol (C), 60 mOsmol kg^–1 sucrose (D) and 26 mOsmol kg^–1 K₄[Fe(CN)₆] (E) or by removing them from the medium. The addition of ethanol, KCl and mannitol to the medium resulted in biphasic responses due to rapid efflux of water, followed by slow influx of solutes. In contrast, addition of sucrose and K₄[Fe(CN)₆] to the external medium gave monophasic responses in which the second phase or solute influx (solute permeation into the root) is missing.

Fig. 6. — Measurement of solute permeabilities (P_sr) and reflection coefficients (σ_sr) of seminal roots of barley for different test solutes using the root pressure probe in well-stirred conditions. Responses of root pressure (P_r) in relation to the change in osmotic pressure in the medium either by adding 100 mOsmol kg^–1 ethanol (A), 56 mOsmol kg^–1 KCl (B), 40 mOsmol kg^–1 mannitol (C), 60 mOsmol kg^–1 sucrose (D) and 26 mOsmol kg^–1 K₄[Fe(CN)₆] (E) or by removing them from the medium. The addition of ethanol, KCl and mannitol to the medium resulted in biphasic responses due to rapid efflux of water, followed by slow influx of solutes. In contrast, addition of sucrose and K₄[Fe(CN)₆] to the external medium gave monophasic responses in which the second phase or solute influx (solute permeation into the root) is missing.