Table 1.
Name | Sequence | Spacer length | MW (kDa) | PI | Charge | GRAVY | |
---|---|---|---|---|---|---|---|
TiBP | RPRENRGRERGL | N/A | 1.4956 | 11.82 | +3 | −2.633 | |
AMP | LKLLKKLLKLLKKL | N/A | 1.6923 | 10.70 | +6 | 0.500 | |
TiBP-Spacer 3-AMP | RPRENRGRERGL | −GGG LKLLKKLLKLLKKL | 3 | 3.3411 | 11.85 | +9 | −0.890 |
TiBP-Spacer 5-AMP | RPRENRGRERGL | GSGGG LKLLKKLLKLLKKL | 5 | 3.4852 | 11.85 | +9 | −0.871 |
Notes: MW, molecular weight; pI, isoelectric point; GRAVY, GRand Average Value of hydropathicitY; and “−”, gap inserted for sequence alignment. Despite chimeric peptide similarity to one another, we observed improved antimicrobial activity with the altered amino acid composition of the longer peptide spacer, Spacer5.