Fig 3. Representation of the control BiFC constructs and expression analysis.

(A) The membrane-associated control (Myr-VC) lacks any extracellular and transmembrane regions, and has the Lck myristoylation sequence (green) preceeded by a myc tag (pink). Sialophorin (SPN) consists of a large highly glycosylated (grey triangles) ectodomain and single transmembrane spanning region. These constructs were fused to either the N- or C-terminal sequence of the Venus YFP protein and contained either an N-terminal HA-tag (blue) or myc-tag (pink). (B) Western blotting of cell lysates from transfected HEK-293T cells with either anti-myc or anti-HA antibodies confirmed the expression of the constructs at the expected molecular weight. Expression of VE-PTP-VC is shown for reference with β-actin used as a loading control.