Fig 6. Comparison of BiFC signals from VE-cadherin co-expressed with VE-PTP and truncation or deletion constructs of VE-PTP.

(A) Pairs of constructs were transfected into HEK-293T cells and the BiFC signal assessed. Bar graphs represent the average BiFC fluorescence intensity from all cells in three images taken from three independent experiments determined as described in the materials and methods. Values are means ± S.E.M; ** P<0.001 by the Mann-Whitney U test; (B) Overlay of plasma membrane staining and BiFC signal. A representative subfield at higher digital zoom showing detail at the single cell level for VE-PTP truncation and deletion mutants with VE-cadherin is shown; Scale bar 20 μm.