Skip to main content
. 2017 Sep 19;12(9):e0185194. doi: 10.1371/journal.pone.0185194

Fig 2. Co-infection of Gouleako goukovirus (GOLV) and RVFV.

Fig 2

(A) C6/36 cells were mock-infected or infected with GOLV (8 MOI) and/or rMP-12 (0.5 MOI) at 28°C. Total RNA was extracted at 72 hpi (left four lanes). Culture supernatants were collected and transferred into either C6/36 cells (middle four lanes) or Vero cells (right four lanes). Total RNA was then extracted at 72 hpi. The presence of L-, M-, and S-segment RNA of GOLV (top panel) or RVFV (bottom panel) was analyzed by Northern blot. (B) Vero or PK15 cells were infected with GOLV (8 MOI) with or without either rMP-12 (0.5 MOI) or rMP12-ΔNSs16/198 (2.0 MOI) at 28°C. Total RNA was extracted at 18 hpi, and the accumulation of viral RNA was analyzed by Northern blot. Top panel: probes to detect negative-sense GOLV L-, M-, and S-segments, Bottom panel: probes to detect negative-sense RVFV L-, M-, and S-segments. GOLV RNA derived from C6/36 cells infected with GOLV (8 MOI) was used for the positive control for GOLV probes on the membrane.