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. 2017 Sep 19;12(9):e0185194. doi: 10.1371/journal.pone.0185194

Fig 3. Minigenome assays with expressions of N and L proteins derived from Arumowot virus (AMTV) or Rift Valley fever phlebovirus (RVFV).

Fig 3

BHK/T7-9 cells were transfected with plasmids expressing M-segment minigenome RNA from either RVFV or AMTV, and those expressing N or L proteins derived from either AMTV or RVFV. The RVFV-M-rLuc and AMTV-M-rLuc represent the M-segment minigenomes derived from RVFV and AMTV, respectively. Cell lysates were collected at 72 hpt, and the ratio of Renilla luciferase (rLuc) activities to firefly luciferase (fLuc) activities derived from pT7-IRES-fLuc (control plasmid) was shown as percentage: i.e., the same type of minigenome without N and L expression was set as 100%. Bars represent means plus standard errors. Asterisks on error bars represent statistically significant increases compared to samples expressing minigenome only (One-way ANOVA **p < 0.01).