Table 1.

H3K9ac, H3K9me3, H3K27me3, and H3K36me3 modification levels by participant characteristics.

Variable	H3K9ac (%)^a				H3K9me3 (%)^a				H3K27me3 (%)^a				H3K36me3 (%)^a
	Obs^b	Mean^c	SE^c	p-value^d	Obs^b	Mean^c	SE^c	p-value^d	Obs^b	Mean^c	SE^c	p-value^d	Obs^b	Mean^c	SE^c	p-value^d
Occupation
Office worker	103	0.23	0.02	0.29	93	0.36	0.05	0.52	114	27.3	1.0	0.04	113	0.56	0.04	0.40
Truck driver	114	0.20	0.02		94	0.40	0.05		117	24.3	1.0		115	0.51	0.04
Sex
Female	76	0.24	0.02	0.15	63	0.40	0.06	0.73	77	23.6	1.3	0.03	75	0.53	0.05	0.84
Male	141	0.20	0.02		124	0.38	0.04		154	26.9	0.9		153	0.54	0.03
Age (quartile)
Q1 [18–27 years]	54	0.20	0.03	0.93	38	0.34	0.08	0.73	57	28.5	1.5	0.16	56	0.61	0.06	0.28
Q2 [28–32 years]	57	0.22	0.03		46	0.34	0.07		60	25.8	1.4		60	0.48	0.05
Q3 [33–37 years]	52	0.22	0.03		50	0.43	0.07		55	24.5	1.5		54	0.56	0.06
Q4 [38–46 years]	54	0.21	0.03		53	0.39	0.07		59	24.3	1.4		58	0.49	0.05
BMI
14.8–24.9 kg/m²	155	0.21	0.02	0.56	126	0.39	0.04	0.41	162	25.6	0.9	0.90	160	0.53	0.03	0.76
25–29.9 kg/m²	52	0.21	0.03		52	0.40	0.07		58	26.2	1.5		57	0.54	0.06
30–32.4 kg/m²	10	0.28	0.06		9	0.17	0.16		11	26.8	3.4		11	0.62	0.13
Smoking status
Never smoker	123	0.23	0.02	0.17	108	0.39	0.05	0.79	131	24.4	1.0	0.03	130	0.52	0.04	0.47
Ever smoker	94	0.19	0.02		79	0.37	0.06		100	27.6	1.1		98	0.56	0.04
Cigarettes smoked on examination day
0	156	0.22	0.02	0.56	137	0.37	0.04	0.82	169	26.1	0.9	0.87	167	0.54	0.03	0.87
1–5	29	0.18	0.03		23	0.42	0.12		30	25.0	2.0		30	0.48	0.07
6–10	25	0.21	0.04		21	0.44	0.09		25	24.5	2.2		24	0.53	0.08
> 10	7	0.18	0.07		6	0.55	0.27		7	25.2	4.1		7	0.53	0.14

Percent of H3 modifications over total H3 content.

Number of non-missing H3K27me3, H3K36me3, H3K9ac, or H3K9me3 observations (obs).

Means and standard errors (SE) of H3K9ac, H3K9me3, H3K27me3, or H3K36me3 measured on two examination days and estimated by marginal means and corresponding standard deviations from bivariate analysis using mixed-effect regression models.

p-values were calculated using mixed-effect regression models (age, BMI, and cigarettes smoked on examination day were modeled as continuous variables).