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. 2016 Oct 27;1:16013. doi: 10.1038/npjregenmed.2016.13

Figure 8.

Figure 8

Ectopic application of HSP60 stimulates wound healing in diabetic mice and stimulates M2 macrophages in human peripheral blood cells. (a) Representative images of skin puncture wounds of db/db mice on the back at 7, 14 and 21 days after the initial injury. Dotted black lines demarcate the wound opening. Bar = 1 mm. (b) Quantification of wound healing in the untreated control, BSA-treated or GroEL-treated wounds over a 21-day test period. Wound size is expressed as a percentage of the initial wound area. The number of wounds and mice used for the treatment and quantification: 12 wounds from 6 mice for the untreated control and 9 wounds from 9 mice for the BS or GroEL-treated. The difference is significant at 14 and 21 days between untreated control and GroEL-treated, or between BSA-treated and GroEL-treated mice (P<0.05 for all). The difference is not significant for all the time points between untreated and BSA treatment. The statistical analysis was carried out by one-way analysis of variance. (c) Human peripheral blood mononuclear cells stimulated with either LPS or GroEL for 24 h are measured for expression of the M2 marker CD163. GroEL significantly increased M2 phase macrophages over LPS (P=0.006). Asterisks indicate a significant difference. BSA, bovine serum albumin; LPS, lipidpolysaccharide.