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. 2017 Feb 25;45(9):5217–5230. doi: 10.1093/nar/gkx151

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Hrq1 and RecQ4 bind a broad range of DNA substrates. (A) Cartoon of the various DNA substrates used in the EMSA assays (not shown: ssDNA). (B) DNA binding by 100 nM Hrq1 (black) and RecQ4 (red). (C) ssDNA binding as a function of helicase concentration. Both helicases bind tightly to poly(dT) ssDNA. (D) Hrq1 and RecQ4 have a binding site size of 20–40 nt. Competition assays were performed using radiolabeled poly(dT) 40mer and a 100-fold molar excess of unlabeled poly(dT) substrates of the indicated lengths. (E and F) DNA binding curves for Hrq1 (E) and RecQ4 (F) for the indicated substrates.