Table 1. Folding parameters for aptamer docking of wt and apoA_STAB obtained by smFRET.

Variant	Mg [mM]	Ade [μM]	ΔG(UD) Hista [kcal/mol]	kdockb [s−1]	kundockb [s−1]	ΔGdockKinc [kcal/mol]
Wt	2	0	0.8 ± 0.1	0.7 ± 0.1	0.98 ± 0.03	0.2 ± 0.1
	20	0	0.2 ± 0.1	0.7 ± 0.1	1.18 ± 0.02	0.3 ± 0.1
	2	100	0.1 ± 0.1	0.7 ± 0.1	1.5 ± 0.2	0.4 ± 0.1
apoASTAB	2	0	0.5 ± 0.1	0.5 ± 0.1	0.9 ± 0.1	0.3 ± 0.1
	20	0	0.2 ± 0.1	0.5 ± 0.1	1.1 ± 0.1	0.5 ± 0.1
	2	100	0.0 ± 0.1	0.49 ± 0.04	1.2 ± 0.3	0.5 ± 0.1

^aFree enthalpy of the docked state relative to the undocked state determined from the FRET distribution observed in the FRET histogram as ΔG(UD) = –RT*ln(D/U). The reported error was calculated from the fitting errors of the FRET populations by Gaussian error propagation.

^bThe reported rate constants are mean values with standard deviations from single-exponentially fitted dwell-time histograms of two independent experiments.

^cFree docking enthalpy determined from the observable kinetics as ΔG_dock = –RT*ln(k_dock/k_undock). The reported error was calculated from the standard deviations of the rate constants from two independent experiments by Gaussian error propagation.