Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Direct interaction between TopA and CobB. Purified recombinant TopA and His-tagged CobB were allowed to interact before pull-down by HisPur Cobalt Agarose Resin. The presence of TopA in the bound proteins eluted from the cobalt resin was visualized by western blot analysis with anti-TopA antibodies (A). The co-eluted His-CobB was stained by Coomassie blue following SDS PAGE (B) or silver on the transfer membrane (C).
