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. 2017 Apr 3;45(9):5309–5322. doi: 10.1093/nar/gkx231

Figure 2.

Figure 2.

PS-ASO cargos bud as ILVs inside LEs in live cells. (A) Representative images of cells overexpressing RAB5(Q79L)-GFP (green) treated with 2 μM Cy3-labeled PS-ASO (red) for 4 h. The nuclei were stained with Hoechst 33342 (blue). Scale bar, 5 μm. (B) Representative merged images of cells overexpressing RAB7a-GFP (green) incubated with 2 μM Cy3-labeled PS-ASO (red) for 4 h followed by treatment with YM201636 for an additional 8 h. The nuclei were stained with Hoechst 33342 (blue). Scale bar, 5 μm. (C) Representative images of cells overexpressing RAB5(Q79L)-GFP (gray) and treated with Cy5-labeled PS-ASO (red) and N-Rh-PE (green) for 4 h. The nuclei were stained with Hoechst 33342 (blue). Scale bar, 5 μm. (D) Representative images of cells overexpressing RAB5(Q79L)-GFP (green) treated with Cy5-labeled-transferrin (red) or Alexa Fluor® 594-LDL (red) for 4 h. The nuclei were stained with Hoechst 33342 (blue). Scale bar, 5 μm.