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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Apr 26;1864(11 Pt A):1940–1951. doi: 10.1016/j.bbamcr.2017.04.015

Fig. 3.

Fig. 3

Cleavage of type V collagen by MMP-12 CAT domain (top) and trypsin (bottom). Type V collagen (333 nM) was treated with 4–32 nM of MMP-12 or 0.5–4 nM of trypsin in 50 mM Tris•HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 0.05% Brij35, 1 μM ZnCl2 overnight at either room temperature (gels on the left) or 37 °C (gels on the right). MMP12 CAT domain or trypsin catalyzed the hydrolysis of type V collagen at 37 °C, but not at room temperature. “Lad” is the molecular weight ladder, “Col V” is type V collagen alone, and “Enzm” is trypsin alone.