Table 2.

Kinetic parameters for human collagen hydrolysis by MMPs.

Collagen type	Enzyme	kcat/KM (sec−1M−1)	KM (μM)	kcat (sec−1)	Assay T (°C)	Reference
I	MMP-1	14,600	0.82	0.012	30	[56]
I	MMP-1	18,750	0.80	0.015	30	[56]
I	MMP-1	525,000	0.9	0.472	37	[243]
Ia	MMP-1	742,000	0.31	0.23	27	[244]
Ib	MMP-1	4,740	1.3	0.00617	27	[74]
Ic	MMP-1	4,610	1.0	0.00461	25	[64]
Ic	MMP-2	529	8.5	0.00450	25	[64]
I	MMP-8	2,540	0.7	0.00178	25	[63]
Ia	MMP-8	2,260,000	0.21	0.470	37	[245]
Ib	MT1-MMP	680	2.9	0.00197	27	[74]
II	MMP-1	132	2.1	0.00028	25	[246]
II	MMP-8	593	1.1	0.00065	25	[63]
III	MMP-1	112,000	1.4	0.157	25	[50]
III	MMP-1	118,000	1.3	0.153	25	[50]
IIId	MMP-1	93,000	15	1.4	25	[247]
IIIe	MMP-1	59,000	1.3	0.08	25	[52]
III	MMP-8	131	1.8	0.00024	25	[63]
IIId	MMP-13	22,600	14	0.32	25	[247]
III	MT1-MMP	366	0.95	0.00035	27	[82]
III	MT3-MMP	1,926	0.45	0.00087	27	[82]

^aBovine type I collagen.

^bGuinea pig type I collagen.

^cRat type I collagen.

^dHuman type III collagen sequence inserted into bacterial collagen.

^eRecombinant type III collagen expressed in P. pastoris.