Figure 1.

³H‐H₂O and ¹⁴C‐palmitate incorporation in Pnpla3^+/+(WT) and Pnpla3^148/M/M (KI) mice. (A‐C) Incorporation of ³H‐H₂O into (A) fatty acids, (B) TG, and (C) PA in liver, white adipose tissue (WAT), kidney, and blood of 11‐ to 13‐week‐old female PNPLA3‐WT and PNPLA3‐KI mice (n = 10 per group). Mice were fed an HSD for 4 weeks and then feeding was synchronized for 3 days (protocol 1). During the last fasting cycle, the fast was extended to 24 hours. Mice were then stagger‐fed at 4‐hour intervals before being injected intraperitoneally with ³H‐H₂O (50 mCi in 250 μL 0.9% NaCl). After 1 hour, the mice were killed and tissues were processed as described in Materials and Methods. Bars represent the mean ± standard error of the mean (SEM). (D) Incorporation of ¹⁴C‐palmitate into hepatic TG in 12‐week old female WT and KI mice (10/group). Mice were handled as described in panel A but were injected through the tail vein with ¹⁴C‐palmitate‐BSA (200 μCi) and killed after 1 hour. Each bar represents the mean ± SEM. Levels were compared among lines using a Student t test. The experiment was repeated once, and the results were similar.