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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Immunol. 2017 Aug 14;199(7):2316–2322. doi: 10.4049/jimmunol.1700719

Figure 5. Effect of LBH deficiency on passive K/BxN arthritis in vivo.

Figure 5

(A) At 7 to 16 weeks of age, male Rosa26Cre-Lbh+/+ (Lbh+/+, n = 16) and Rosa26Cre-Lbhloxp/loxp (Lbh-/-, n = 13) mice were injected i.p. with K/BxN sera to induce arthritis on days 0 and 2. Clinical score and ankle swelling was measured every other day after serum transfer until day 12. Mean ± SEM is shown. Data were analyzed using 2-way ANOVA. (B) Relative expression of mRNA for Il1β, Il6, Mmp3 genes. Lbh+/+ (n = 7) and Lbh-/- (n = 6) mice were administered K/BxN serum, and after 12 days, the ankle joints were harvested and homogenized, and mRNA expression was analyzed by quantitative polymerase chain reaction. Values are mean ± SEM. Data were analyzed using the 2-tailed unpaired t test. (C) Western blots for phospho-CHK1 (S345) and total CHK1 in ankle joints of Lbh+/+ (n = 7) and Lbh-/- (n = 6) mice. Bars represent the average. Values are the mean ± SEM. Data were analyzed using the 2-tailed unpaired t test.