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. Author manuscript; available in PMC: 2018 Sep 19.
Published in final edited form as: Biochemistry. 2017 Sep 6;56(37):4940–4950. doi: 10.1021/acs.biochem.7b00695

Figure 11.

Figure 11

NH2-CAM concentration dependence of the overall reaction time course. The solid curves are for optical changes at 480 nm from the reaction of CmlIred (200 μM) with 1-16 mM NH2-CAM (final, postmix concentrations) in O2-saturated buffer and the dashed curves are for simulation of the same reaction using numerical integration. The rate constants and reactions used for the simulation are shown in Scheme 4 (see Discussion). The postmix O2 concentration is ∼ 0.9 mM at 4 °C. (50 mM Bicine at pH 9 and 4 °C).