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. 2017 Sep 19;7:179. doi: 10.1186/s13568-017-0477-z

Fig. 3.

Fig. 3

A comparison of DGGE profiles of PCR amplified bacterial 16S rRNA gene for the specimen No: 1. DNA was extracted using six different DNA extraction methods using 25 mg of wound tissue debridement specimen no. 1. Bacterial fingerprinting profile is based on 30–55% denaturing gradient. “L” lanes represent the in house bacterial reference panel which includes S. aureus, Acinetobacter spp, Group B Streptococcus spp., E. faecalis, Group A Streptococcus spp. and E. coli from top to bottom respectively. Other lanes show bacterial fingerprinting profile of each extraction method in duplicate (a, b) for the specimen No. 1, collected from a subject with a chronic wound