Table 2.
Patient/specimen No. | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
01 | 02 | 03 | 04 | 05 | 06 | 07 | 08 | 09 | 10 | |
1 STES buffer and phenol chloroform extraction method (BP1) | ||||||||||
DNA yield (ng/µl) | 48.65 ± 25.24 | 1747.95 ± 404.25 | 480.2 ± 359.07 | 195.25 ± 29.06 | 93.20 ± 31.25 | 937.75 ± 255.90 | 900.30 ± 639.79 | 1096.9 ± 451.7 | 226 ± 83.43 | 69.50 ± 10.89 |
A260/280 | 1.98 ± 0.08 | 1.80 ± 0.01 | 1.82 ± 0.06 | 1.88 ± 0.00 | 1.99 ± 0.02 | 1.88 ± 0.01 | 1.80 ± 0.01 | 1.82 ± 0.01 | 1.83 ± 0.02 | 1.96 ± 0.02 |
A260/230 | 0.87 ± 0.05 | 2.19 ± 0.07 | 1.75 ± 0.35 | 1.27 ± 0.02 | 1.05 ± 0.27 | 2.15 ± 0.01 | 1.82 ± 0.40 | 2.1 ± 0.11 | 1.69 ± 0.04 | 0.77 ± 0.05 |
2 TN150 buffer and phenol chloroform extraction method (BP2) | ||||||||||
DNA yield (ng/µl) | 10.9 ± 1.56 | 310.05 ± 160.16 | 340.6 ± 12.45 | 88.35 ± 27.51 | 46.20 ± 21.64 | 274.05 ± 43.20 | 661.45 ± 500.70 | 402.25 ± 1.91 | 90.25 ± 76.44 | 93.70 ± 42.99 |
A260/280 | 2.07 ± 0.04 | 1.8 ± 0.03 | 1.77 ± 0.00 | 1.77 ± 0.04 | 1.73 ± 0.15 | 1.71 ± 0.02 | 1.86 ± 0.99 | 1.82 ± 0.01 | 1.8 ± 0.06 | 1.84 ± 0.12 |
A260/230 | 0.52 ± 0.01 | 1.84 ± 0.16 | 1.49 ± 0.10 | 1.15 ± 0.30 | 0.69 ± 0.17 | 1.57 ± 0.08 | 1.66 ± 0.32 | 1.79 ± 0.08 | 1.3 ± 0.44 | 1.37 ± 0.36 |
3 DNeasy blood and tissue kit (K) | ||||||||||
DNA yield (ng/µl) | 39.45 ± 21.01 | 400.55 ± 447.25 | 698.2 ± 126.99 | 51.8 ± 28.56 | 50.75 ± 66.39 | 152.65 ± 13.08 | 718.1 ± 608.54 | 80.95 ± 0.49 | 316.25 ± 127.77 | 388.40 ± 120.49 |
A260/280 | 1.87 ± 0.05 | 1.80 ± 0.01 | 1.81 ± 0.01 | 1.78 ± 0.11 | 1.87 ± 0.17 | 1.80 ± 0.01 | 1.82 ± 0.01 | 1.8 ± 0.01 | 1.81 ± 0 | 1.84 ± 0.01 |
A260/230 | 2.1 ± 0.40 | 1.74 ± 0.66 | 2.17 ± 0.11 | 2.19 ± 0.06 | 1.56 ± 0.00 | 1.65 ± 0.37 | 2.15 ± 0.07 | 1.81 ± 0.11 | 1.82 ± 0.55 | 2.15 ± 0.06 |
4 Heating in distilled water (H1) | ||||||||||
DNA yield (ng/µl) | 213.25 ± 46.46 | 51.65 ± 19.02 | 260.95 ± 70.36 | 72.2 ± 36.63 | 142.45 ± 47.73 | 277.6 ± 9.76 | 429.05 ± 4.60 | 145.6 ± 168.57 | 97.25 ± 73.89 | 255.35 ± 109.95 |
A260/280 | 1.22 ± 0.04 | 1.38 ± 0.06 | 1.26 ± 0.01 | 1.56 ± 0.05 | 1.36 ± 0.17 | 1.39 ± 0.05 | 1.41 ± 0.05 | 1.39 ± 0.05 | 1.41 ± 0.09 | 1.74 ± 0.18 |
A260/230 | 0.3 ± 0.04 | 0.28 ± 0.02 | 0.43 ± 0.00 | 0.56 ± 0.07 | 0.29 ± 0.02 | 0.32 ± 0.01 | 0.56 ± 1.01 | 0.33 ± 0.11 | 0.25 ± 0.04 | 0.71 ± 0.36 |
5 Heating in aqueous NaOH (H2) | ||||||||||
DNA yield (ng/µl) | 131 ± 25.74 | 356.9 ± 189.22 | 606.95 ± 82.94 | 185.5 ± 32.81 | 146.8 ± 40.45 | 386.15 ± 96.1 | 184.25 ± 70.22 | 240.65 ± 189.86 | 210.05 ± 28.5 | 208.6 ± 113.14 |
A260/280 | 1.08 ± 0.04 | 1.37 ± 0.04 | 1.51 ± 0.08 | 1.38 ± 0.05 | 1.15 ± 0.04 | 1.31 ± 0.08 | 1.46 ± 0.02 | 1.18 ± 0.13 | 1.1 ± 0.01 | 1.47 ± 0.03 |
A260/230 | 0.25 ± 0.03 | 0.46 ± 0.04 | 0.79 ± 0.13 | 0.48 ± 0.06 | 0.26 ± 0.07 | 0.27 ± 0.02 | 0.57 ± 0.00 | 0.25 ± 0.08 | 0.3 ± 0.02 | 0.39 ± 0.01 |
6 Salting out method (S) | ||||||||||
DNA yield (ng/µl) | 62.2 ± 26.87 | 1701.8 ± 1046.80 | 3599.4 ± 1363.87 | 327.5 ± 286.38 | 118.45 ± 23.83 | 581 ± 50.91 | 3264.4 ± 3135.45 | 1169.75 ± 582.16 | 246.85 ± 24.96 | 1248.4 ± 792.67 |
A260/280 | 1.95 ± 0.04 | 1.81 ± 0.07 | 1.81 ± 0.04 | 1.97 ± 0.13 | 1.95 ± 0.16 | 1.78 ± 0.04 | 1.84 ± 0.06 | 1.83 ± 0.4 | 1.83 ± 0.04 | 1.83 ± 0.11 |
A260/230 | 0.9 ± 0.51 | 0.61 ± 0.3 | 0.96 ± 0.01 | 0.37 ± 0.41 | 0.15 ± 0.04 | 0.68 ± 0.03 | 0.77 ± 0.40 | 0.48 ± 0.25 | 0.58 ± 0.11 | 0.46 ± 0.29 |