Fig. 7.

Percent FL change of various ICG NC formulations in the presence of 5 mM phosphate and 4 wt. % ($40\mathrm{mg}/\mathrm{mL}$) BSA. [▪ ${\mathrm{C}}_4$ in situ complex, • ${\mathrm{C}}_8$ in situ complex, ▾ ${\mathrm{C}}_8$ premade complex (two replicates shown), ▴ ${\mathrm{C}}_{12}$ in situ complex (two replicates shown)]. The ${\mathrm{C}}_4$ complex showed the greatest change in FL indicating the greatest amount of ICG exchanged away, as expected, while the ${\mathrm{C}}_8$ counter-ion provided the best protection.