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. 2017 Sep 19;7:11858. doi: 10.1038/s41598-017-12195-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spermatogonia related marker proteins’ expression in bisphenol A (BPA)-treated cultured germ cells enriched for spermatogonial stem cells. Immunostaining of undifferentiated spermatogonia markers’ expression in 1 week BPA-administrated cultured germ cells are indicated as red fluorescence, (A) PLZF, (B) GFRα1 and (C) RET. Pictures of low BPA treatment (0.1 µM BPA) and high BPA treatment (100 µM BPA) are given because the expression patterns of all treatment groups are visually similar. (E, F, and G) The percentage of these markers’ expression among total cells showed sequentially as bar graph. (D) The expression of differentiated spermatogonia specific marker C-KIT in 1 week BPA-administrated cultured germ cells (red). (H) Bar graph presentation of the percentage of C-KIT positive cells. Data were obtained from 6 independently established cultures with different BPA treatments. Cells were counterstained with DAPI (blue). Scale bars = 50 μm.