Table 2.

Summary statistics of MS-CA performed by generalized linear model (Vitamin B₁₂ vs. control and MTX). Null deviance was 157 on 15 degrees of freedom and AIC was 73.8.

	Value	Standard error	t-value	p-value
Intercept	10.96	1.82	6.03	<<0.001
Methotrexate	−7.28	1.42	−5.11	<<0.001
Chlamy-control	−3.75	1.42	−2.63	0.023
Chlorella-control	−3.31	1.42	−2.32	0.040
Experiment	−1.24	1.01	−1.23	0.24

glm revealed that MTX exposed animals display a 7.28% decrease in global DNA methylation compared to Daphnia supplemented with 1.5 mg/L vitamin B₁₂. A similar trend was observed in control animals fed with Chlamydomonas (Chlamy) and Chlorella (with decreases of 3.78% and 3.31% in DNA methylation, respectively, relative to vitamin B₁₂).