Table 1.
Six potential mRNA targets for miR-9a in the testis
| Gene | 1-day young | 30-days aged | Seeds | ||||||
|---|---|---|---|---|---|---|---|---|---|
| logFC | Significance (P-value) | Average CPM per gene | logFC | Significance (P-value) | Average CPM per gene | ||||
| WT | Mir-9a mutant | WT | Mir-9a mutant | ||||||
| N-cad herin | 1.6 | 1.3E−26 | 20.7 | 63.4 | 0.9 | 4.9E−08 | 27.0 | 49.3 | 2 |
| sticks and stones | 1.2 | 1.08E−05 | 3.08 | 7.1 | 1.0 | 0.0005 | 2.57 | 5.23 | 1 |
| CG10512 | 1.5 | 5.25E−25 | 23.56 | 67.0 | 1.5 | 1.5E−22 | 41.29 | 115.62 | 1 |
| lame duck | 2.0 | 1.26E−09 | 1.02 | 4.2 | 1.1 | 0.0009 | 1.6 | 3.43 | 1 |
| meso18e | 1.3 | 7.35E−11 | 9.79 | 23.6 | 1.0 | 1.6E−6 | 11.9 | 23.5 | 1 |
| CG34136 | 1.5 | 0.0002 | 1.03 | 3.0 | 2.2 | 1.6E−6 | 0.7 | 3.1 | 1 |
| Senseless | 1.0 | 0.0001 | 3.8 | 7.7 | 1.0 | 0.001 | 3.5 | 6.4 | 1 |
| E-cad herin | 0.1 | 0.453 | 54.5 | 59.3 | 0 | 0.95 | 63.4 | 64.1 | 0 |
Transcriptome filtration based on logFC ≥ 0.9, significance cutoff (P-value ≤ 0.05), and minimal reading levels (sum reads per each gene ≥ 1) obtained a group of 231 genes that showed higher expression in mir-9a[E39] mutant vs. control in either young or aged testis. Comparison of this list to the predicted miR-9a targets resulted in the presented six candidates. The negative control, E-cad, is shown at the bottom of the table.