Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 19;7:11645. doi: 10.1038/s41598-017-12004-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Manganese stress evokes ROS production. (a) (i) The FACS data shows the changes in fluorescence intensity of H₂DCFDA, an indicator dye of oxidative stress, under manganese stress and iron supplementation. Fluorescence intensity is directly proportional to the amount of ROS species in the cell. (ii) Normalized MFI values from three independent experiments are shown by bar diagrams. (b) The normalized MFI values of H₂DCFDA dye under manganese stress and in the presence of ROS quenchers (Tr: tiron, SP: sodium pyruvate, TU: thiourea). (c) The H₂DCFDA fluorescence values were normalized by intracellular dye contents to show that manganese stress evokes ROS production. (d) To validate H₂DCFDA fluorescence results, another dye DHR123 has been used. Increased intracellular dye-normalized fluorescence values of DHR suggests that manganese-fed cells evoke ROS. (e) The fluorescence values of DHR123 in the bar diagram indicates that manganese-fed cells produces elevated level of H₂O₂ in comparison to the un-fed cells at 30 minutes and 60 minutes time points. Iron-supplemented cells marginally suppressed H₂O₂ production. Data are means ± SD (n = 3); *P < 0.001, paired T-test.