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. 2017 Sep 19;7:11834. doi: 10.1038/s41598-017-10738-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Centrifuged sucrose gradients of the new iridescent virus in natural light (a) and in red laser beam (b); Fractions of the gradients were taken and measured for buoyant density (pink) and the optical density at 280 nm (dark purple), (c). Four bands were pointed by the arrows and the 3^rd band was virions. TEM visualization of the negatively stained purification of the new iridescent virus. (d) Virions pelleted through a 30% (w/w) sucrose solution; (e), (f), and (g) Virions purified in the sucrose gradients.