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. 2017 Sep 19;7:11834. doi: 10.1038/s41598-017-10738-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ISH using a digoxigenin labeled 279 bp probe for the shrimp hemocyte iridescent virus on histological sections of L. vannamei. (a)–(d) hematopoietic tissue, gills, hepatopancreas and periopods in SHIV-positive sample, respectively; (e)–(h) hematopoietic tissue, gills, hepatopancreas and periopods in SHIV-negative sample, respectively. In (a)–(d), blue signals were observed in the cytoplasm of the hemocytes of hematopoietic tissue, gills, sinus of hepatopancreas and periopods. In (e)–(h), no hybridization signal was seen on the same tissues of SHIV-negative L. vannamei except some non-specific signals on cuticle. Bar, 10 μm (a and b), 20 μm (c and d), and 50 μm (e–h), respectively.