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. 2017 Sep 19;7:11834. doi: 10.1038/s41598-017-10738-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of the shrimp hemocyte iridescent virus with the nested PCR method. (a) Specificity analysis of the nested PCR method. M, DL500 molecular mass marker; Lanes 1–6: PCR amplified products with DNA template samples extracted from health L. vannamei and L. vannamei infected with WSSV, IHHNV, HPV, AHPND, and EHP, respectively; lane 7: PCR amplified products with DNA template sample extracted from shrimp infected with the iridescent virus; and lane 8: negative control. (b) Sensitivity test of the nested PCR method. M, DL1000 molecular mass marker; Lane 1: negative control; Lines 2–10: PCR amplified products with the diluted DNA solutions (10⁰–10⁻⁸) of L. vannamei naturally infected with the shrimp iridescent virus.