Figure 3.

Schematic organization of the genome of pigeonpea sterility mosaic viruses: (A) PPSMV-I contains four (-) sense single strand RNA segments and (B) PPSMV-II contains six genomic RNA segments. The large RNA-1 encodes RdRp, RNA-2 encoding glycoprotein precursor (GP) and the cleavage site was denoted by gray triangle, RNA-3 encodes nucleocapsid protein and RNA-4 encodes movement protein. PPSMV-II contains additional RNA-5 (55 kDa), RNA-6 (27 kDa) with unknown function. Endonuclease and probable cap binding sites are denoted in orange and white boxes of the RdRp segments of the PPSMVs. The polymerase catalytic motifs A to F were identified in bunya-RdRp central region coloured as green box. Catalytically active aspartate (Asp₁₂₅₆) of SDD motif was marked by a black triangle. The RNAs are shown as black and blue boxes and the encoded proteins as grey boxes. (C) PPSMV-I and PPSMV-II RdRps are being alike and the line drawing representation is of PPSMV-II which shows imaginary SRD-1, SRD-2 and SRD-3 domains and the location of endonuclease (orange), Bunya RdRp (green) and proposed cap binding site (blue) subunits (See Table 1).