Table 2.

Comparison of percent amino acid identities of genomic proteins pigeonpea sterility mosaic viruses and between the members of genus Emaravirus. P1 (RdRp), P2 (GP), P3 (NCP), P4 (MP), P5 and P6 proteins.

Pigeonpea sterility mosaic virus-I genome (PPSMV-I Patancheru sub- isolate Chevella)
Virus	P-1	P-2	P-3	P-4	P-5	P-6
PPSMV-I*	100	100	100	100
PPSMV-1(P)**	97.04	98.15	99.68	100
RRV	54.24	43.97	41.50	38.55
PPSMV-II*	53.92	44.18	41.58	40.78
PPSMV-2(P)	53.83	43.87	41.58	40.78
FMV	53.37	45.24	41.58	39.39
EMARaV	48.33	39.05	35.74	14.52
RYRSaV	47.98	40	33.44	27.53
RLBV	33.95	28.72	25.70	20.39
WMoV	32.55	24.72	22.90	18.29
AcCRaV	48.47	40.50	35.95	29.58
Pigeonpea sterility mosaic virus -II genome (PPSMV-I Patancheru sub- isolate Chevella)
EMARaV	49.69	35.76	35.76	19.68	—	—
RYRSaV	46.91	39.37	38.03	32.96	21.53	—
PPSMV-I#	53.92	44.18	41.58	40.78	—	—
PPSMV-1(P)	53.65	44.03	41.58	40.78	86.47	—
RRV	68.38	52.26	61.86	60.94	—	—
FMV	73.75	57.72	79.94	74.70	32.77	31.72
PPSMV-II#	100	100	100	100	100	100
PPSMV-2(P)	99.61	98.00	98.40	99.45	94.93	97.90
RLBV	34.70	24.84	21.79	17.37	16.16	17.71
WMoV	33.64	23.54	20.37	21.41	19.02	17.01
AcCRaV	47.44	40.09	38.82	31.34	26.53	—

PPSMV-1P** (Patancheru isolate) contains more than four RNA segments and PPSMV-I (Patancheru sub-isolate Chevella) genome was compared with the first four functional RNAs of other emaraviruses. PPSMV-1P** and PPSMV-I* appears to be almost identical. PPSMV-I has closest similarity to RRV followed by PPSMV-II and PPSMV-2P (Patancheru isolate). **In our study, we disputed the claim that PPSMV-1P has five genomic segments. PPSMV-2P and PPSMV-II^# (Patancheru sub-isolate Chevella) appears to be almost identical. PPSMV-II^# is closely related to FMV in all corresponding RNA segments including RNA-5, followed by RRV, in comparison with other emaraviruses. RLBV and WMoV contain largest genomes with eight RNA segments and we have included the first six of them for the analysis.