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. 2017 Sep 19;8:605. doi: 10.1038/s41467-017-00258-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Rosa26 ^CreER-mediated clonal analysis reveals heterogeneous contribution of E9.5 progenitors to pancreas organogenesis. a Schematic overview of lineage relationships based on previous global lineage tracing. b Schematic overview of strategy applied to identify fates of clonal progeny from E9.5 pancreatic progenitors. c 3D maximum intensity projection (MIP) of a large, multipotent clone containing acinar (CPA1), progenitors lining the ducts (SOX9) and endocrine progeny (PAX6, from other multipotent clone) d. Scale bars, 100 µm c and 15 µm d. e 3D MIP of a bipotent clone containing endocrine (insets 1 and 2) and ductal (inset 3) clonal progeny f. Scale bars, 100 µm e and 10 µm f. g 3D MIP and optical section (inset) showing a single-labelled endocrine cell after clonal analysis from E9.5 to E14.5. Note the localisation of the GFP⁺ cell in an E-CAD^Low endocrine cluster. Scale bars, 80 µm and 15 µm (inset). h Quantification of clone sizes and compositions following clonal analysis from E9.5 to E14.5 (n = 170 embryos, 20 with clones, 1 excluded due to poor immunocytochemistry-the images displayed show representative data from those)