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. 2017 Sep 19;7:11878. doi: 10.1038/s41598-017-12183-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Systemic treatment with bumetanide during early development altered KCC2 and GABA_A receptor subunit expression in adolescent rats after maternal separation. (A–E) NKCC1 (A), KCC2 (B), GABA_A receptor α1 subunit (C), GABA_A receptor α5 subunit (D), and GABA_A receptor β2,3 subunit (E) levels in the CA1 area of the hippocampus after maternal separation and bumetanide treatment. (F) Representative Western blots in the CA1. Full-length blots are presented in Supplementary Figure S6. n = 12 per group (6 females, 6 males). The data are expressed as mean ± SEM. *p < 0.05.