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. 2017 Sep 19;7:11893. doi: 10.1038/s41598-017-12131-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CISD2 expression affects cell apoptosis. (A) Cell apoptosis assay using double staining technique. Cells were seeded overnight, and then labeled with annexin V and PI for flow cytometry analysis. (B) Western blot analysis of cell apoptosis markers in shCISD2 transfectant cells and their vector control cells. (C) Cell apoptosis in the absence or presence of cisplatin. Cells were seeded into six-well plates and incubated overnight, then cultured in serum-free media with or without cisplatin (30 μM). Cells were harvested at indicated time interval, labeled with annexin V and PI for flow cytometry assay. Data are mean ± SD of triplicate measurements. **P < 0.01, Student’s t-test.