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. 2017 Sep 19;7:11893. doi: 10.1038/s41598-017-12131-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CISD2 promotes cell invasion in lung ADC cells. (A and B) Left panel, representative images of cell invasion assay showing invading cells on the bottom side of membrane of transwells; right panel, result of quantification analysis. (C) RT-qPCR analyses of the transcript levels of EMT markers in shCISD2 transfectant cells. (D) Western blot analysis of EMT marker proteins in shCISD2 transfectant cells. (E) RT-qPCR analyses of the transcript levels of EMT markers in CISD2(+)-CL1-1 cell. (F) Western blot analysis of EMT markers in CISD2(+)-CL1-1 cells. For (A), (B), (C) and (E), p values were obtained using Student’s t-test, *P < 0.05, **P < 0.01. All data are mean ± SD of at least triplicate measurements.